Induction of triacylglycerol production in Chlamydomonas reinhardtii: comparative analysis of different element regimes.

Bioresour Technol. 2014 Mar;155:379-87. Çakmak ZE1, Ölmez TT2, Çakmak T3, Menemen Y1, Tekinay T4.

Show Affiliations

1Department of Biology, Faculty of Arts and Sciences, Kırıkkale University, 71450 Kırıkkale, Turkey.and

2UNAM, Institute of Materials Science and Nanotechnology, Bilkent University, 06800 Ankara, Turkey.and

3Department of Molecular Biology and Genetics, Faculty of Science, Istanbul Medeniyet University, 34730 Istanbul, Turkey.and

4Gazi University, Life Sciences Application and Research Center, 06830 Ankara, Turkey; Gazi University, Polatlı Science and Literature Faculty, 06900 Ankara, Turkey. Electronic address: [email protected]

Abstract

In this study, impacts of different element absence (nitrogen, sulfur, phosphorus and magnesium) and supplementation (nitrogen and zinc) on element uptake and triacylglycerol production was followed in wild type Chlamydomonas reinhardtii CC-124 strain. Macro- and microelement composition of C. reinhardtii greatly differed under element regimes studied. In particular, heavy metal quotas of the microalgae increased strikingly under zinc supplementation. Growth was suppressed, cell biovolume, carbohydrate, total neutral lipid and triacylglycerol levels increased when microalgae were incubated under these element regimes. Most of the intracellular space was occupied by lipid bodies under all nutrient starvations, as observed by confocal microscopy and transmission electron micrographs. Results suggest that sulfur, magnesium and phosphorus deprivations are superior to nitrogen deprivation for the induction triacylglycerol production in C. reinhardtii. On the other hand, FAME profiles of the nitrogen, sulfur and phosphorus deprived cells were found to meet the requirements of international standards for biodiesel.

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Induction of triacylglycerol production in Chlamydomonas reinhardtii: comparative analysis of different element regimes. .Renewable Energy Global Innovations

 

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