Effective harvesting of the microalgae Chlorella vulgaris via flocculation–flotation with bioflocculant

Journal Reference

Bioresource Technology, Volume 198, December 2015, Pages 922–925.

Xueqian Lei1, Yao Chen1, Zongze Shao2, Zhangran Chen1, Yi Li1, Hong Zhu1, Jingyan Zhang1, Wei Zheng1, 3, Tianling Zheng1, ,  

Show Affiliations
  1. State Key Laboratory of Marine Environmental Science and Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, School of Life Sciences, Xiamen University, Xiamen, China
  2. Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, SOA; Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Xiamen 361005, China
  3. State Environmental Protection Key Laboratory of Microorganism Application and Risk Control (SMARC), Tsinghua University, 100084 Beijing, China


In this study, bioflocculant from Cobetia marina L03 could be used for effective harvesting of the microalgae Chlorella vulgaris via flocculation–flotation. A flotation efficiency of 92.7% was observed when 20 mg L−1 bioflocculant was tested for flocculating the microalgal cells with 5 mM CaCl2. The bioflocculant was stable at wide ranges of pH and temperature, which is advantageous for its application under various conditions. Chemical analysis of the bioflocculant indicated that it is composed of 31.6% total sugar and 0.2% protein (w/w). This bioflocculant has potential for the high-efficiency harvesting of microalgae and may be useful in reducing one of the barriers to microalgal biofuel production.

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