Scientists from the Hangzhou Dianzi University (China) have devised an efficient combination bioprocess of solid state fermentation (SSF) and dark fermentation to produce hydrogen from food waste. Aspergillus awamori and Aspergillus oryzae were utilized in SSF from food waste to generate glucoamylase and protease which were used to hydrolyze the food waste suspension to get the nutrients-rich (glucose and free amino nitrogen (FAN)) hydrolysate. The food waste hydrolysate was then used as substrate for hydrogen production in a continuous stirred mixed immobilized reactor (CMISR). Obviously, more hydrogen yield could be achieved using glucose solution (food waste hydrolysate) rather than direct food waste solid as substrate. Moreover, the fermentability of food waste could be improved by enzymatic hydrolysis that increases the accessibility of the starch in the food waste hydrolysate. The results are very positive and demonstrated that the proposed combination bioprocess could effectively accelerate the hydrolysis rate, improve raw material utilization and enhance hydrogen yield.
Bioresour Technol. 2015 Mar;180:54-8. Han W1, Liu da N2, Shi YW1, Tang JH3, Li YF4, Ren NQ5.Show Affiliations
1College of Materials and Environmental Engineering, Hangzhou Dianzi University, Hangzhou 310018, China.and
2Department of Endocrinology, The First Hospital of Harbin, Harbin 150001, China.and
3College of Materials and Environmental Engineering, Hangzhou Dianzi University, Hangzhou 310018, China. Electronic address: [email protected]
4School of Forestry, Northeast Forestry University, Harbin 150060, China; State Key Lab of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China.and
5State Key Lab of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China.
A continuous mixed immobilized sludge reactor (CMISR) using activated carbon as support carrier for dark fermentative hydrogen production from enzymatic hydrolyzed food waste was developed. The effects of immobilized sludge packing ratio (10-20%, v/v) and substrate loading rate (OLR) (8-40kg/m(3)/d) on biohydrogen production were examined, respectively. The hydrogen production rates (HPRs) with packing ratio of 15% were significantly higher than the results obtained from packing ratio of 10% and 20%. The best HPR of 353.9ml/h/L was obtained at the condition of packing ratio=15% and OLR=40kg/m(3)/d. The Minitab was used to elicit the effects of OLR and packing ratio on HPR (Y) which could be expressed as Y=5.31 OLR+296 packing ratio+40.3 (p=0.003). However, the highest hydrogen yield (85.6ml/g food waste) was happened at OLR of 16kg/m(3)/d because of H2 partial pressure and oxidization/reduction of NADH.
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