On-site enzymes produced from Trichoderma reesei RUT-C30 and Aspergillus saccharolyticus for hydrolysis of wet exploded corn stover and loblolly pine.

Bioresour Technol. 2014 ;154:282-9.

Vandana Rana, Anahita D. Eckard, Phillip Teller and Birgitte K. Ahring.

Bioproducts, Sciences and Engineering Laboratory (BSEL), Washington State University Richland, WA 99354, United States.



Cellulase production by two filamentous fungi Trichoderma reesei RUT-C30 and novel fungal strain, Aspergillus saccharolyticus on pretreated corn stover was investigated. Cellulase production was followed by the hydrolysis of two feedstocks, wet-exploded corn stover (WECS) and wet-exploded loblolly pine (WELP) by on-site produced enzyme cocktails containing cellulase from T. reesei RUT-C30 and {Beta} -glucosidase from A. saccharolyticus. The sugar yields using the on-site enzyme cocktails were compared with commercial enzymes preparations, Celluclast 1.5L and Novozym 188 at two substrate concentrations, 5% and 10% (w/w) and enzyme loading at 5 and 15 FPU/g glucan for WECS and WELP. The highest sugar yields were obtained at 5% (w/w) substrate concentration and 15 FPU/g glucan for both feedstocks. Glucose yields of 81% and 88% were obtained from on-site and commercial enzymes, respectively using

WECS as feed stock. The sugar yields were 55% and 58% for WELP samples hydrolyzed with on-site and commercial enzymes, respectively.


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Additional Information

High cost of enzymes is one of the toughest barriers in the commercialization of biofuels. With the intent of enabling cost-efficient conversion of recalcitrant lignocellulosic biomass to biofuels, we developed a method of producing on-site enzymes for cellulose conversion. Previously published studies on production of on-site enzymes from different fungal strains were limited to i) lower enzyme concentration yields or ii) expensive growth medium was used. In contrast to previous work, this study focused on growing fungi directly in concentrated biomass media using the mesophilic fungi Trichoderma reesei Rut C-30 and Aspergillus saccharolyticus (novel strain) for  cellulase and {Beta}-glucosidase production, respectively. The enzymes were tested at low concentrations (5-15 FPU/g glucan) for hydrolysis of pretreated loblolly pine and corn stover (5-10% dry matter). Comparable glucose yields were obtained from on-site produced and commercial enzymes (Celluclast 1.5L + Novozym 188). On-site produced enzymes from T. reesei and A. saccharolyticus open up the possibility of converting a wide range of recalcitrant biomasses to premium grade transportion fuels at comparatively lower cost.


Figure Legend:  On-site enzymes produced in a bioreactor


On-site enzymes produced from Trichoderma .Renewable Energy Global Innovations

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